An alternative way of treating cancer

Using the body’s own controlling processes
as described in Chinese acupuncture,
expressed as peptides in the blood.


Are S. Thoresen DVM
Leikvollgata 31
3213 Sandefjord
Norway
arethore@online.no

#

Sergio Manzetti, M.Sc.
Flåm
5743 Flåm
Norway
Sergio.manzetti@gmail.com



Abstract

In 1975 Thoresen observed and discovered a method of treating breast cancer and other types of cancer with acupuncture. The method has been applied for over 25 years, on humans and dogs. In 2006 Thoresen and Manzetti identified in the blood of a woman with breast cancer the biochemical components that are responsible for the healing stimulated by the acupuncture treatment. The components, which are fragments of known and less known proteins, were tested on three breast cancer cell types, including a new and aggressive drug-resistant cell-type. The results have shown that the peptides in mixture have a stronger effect after only 24 hours than the chief-remedy in modern breast cancer therapy, Tamoxifen, in inhibiting cell-growth of three cell types, including a Tamoxifen-resistant strain. The results also showed that the peptides are not toxic even at a concentration 100-time higher than the toxic Tamoxifen. The acupuncture-induced peptides are the first unknown factors isolated after acupuncture stimulus, and their composition is entirely novel. Their human origin makes them more specific than today’s’ synthetic drugs and can not only prove to be better remedies than conventional drugs, but also prove to be a new way of discovering human remedies to human diseases. This new method relies on extracting knowledge from age-old acupuncture and translate it to factual medicines and better remedies and has been patented and is under publication.



Contents



History and introduction




Thoresens theory and observations on cancer.




Are Simeon Thoresen i skogen

Figure 1; the controlling sequence of the processes of the body according to acupuncture.

On this basis and with this method we will now investigate the effect on cancer, both clinically (Part I) and scientifically (Part II).


PART I

Scientific investigation


University of Massachusetts logo

UNIVERSITY OF MASSACHUSETTS
AMHERST

N235 Morill 1
639 North Pleasant street
Amherst, MA 01003-9298

Department of Veterinary
and Animal Sciences

Kathleen F. Arcaro, Ph.D.
Assistant Professor
Phone: (413) 577 1823
Fax (413) 545 5731
karcaro@nre.umass.edu



Report on Peptides (December 07) tested in the Laboratory of Kathleen F. Arcaro, University of Massachusetts at Amherst, MA


Introduction

During the period of December 13th through December 22, 2006 we prepared and tested 12 peptides in proliferation assays using three human breast cancer cell lines; T47D, MCF-7 and TMX2-28. The T47D and MCF-7 breast cancer cell lines were obtained from the American Type Culture Collection (ATCC) and details of the cell lines and conditions for their maintenance can be obtained from the ATCC website ( http://www.atcc.org/). The TMX2-28 cell line is a Tamoxifen-selected derivative of MCF-7, which lacks the oestrogen receptor, and is characterized by aggressive and invasive growth (see Gozgit et al., 2006 for details).

All following sections where written based on the results gained at University of Massachusetts. The written text is mixed with other parts of the study that may not derivate from the original document from the University of Massachusetts. The original document can be retrieved at request from the authors of this study after publication.


Results

Because of publication being underway we have to limit this section to an abstract given the Journals request. However, we will update this document as soon as possible.

This first-time in history isolation of novel components of a healing process stimulated by acupuncture has been patented and is under publication. The value of a novel remedy for breast cancer of human origin has not been calculated yet, however several companies are investing between 500-2000 million USD in the production and marketing of drugs of synthetic origin. This may raise the value of these human-originated bioactive peptides to a minimum of this value.

Figures below indicate the effect of the peptide mixture on the three breast cancer cell lines. Not the highest effect on the most aggressive and drug-resistant TMX2-28. The peptide mixture as also been tested for cytotoxicity, see figures 4 and 5.

Fig1: Bioactive Peptide mixture cell-growth inhibiting effect on TMX2-28 (Tamoxifen resistant) cell lines.

Fig1: Bioactive Peptide mixture cell-growth inhibiting effect on TMX2-28 (Tamoxifen resistant) cell lines.


Fig2: Bioactive Peptide mixture cell-growth inhibiting effect on MCF-7 cell lines.

Fig2: Bioactive Peptide mixture cell-growth inhibiting effect on MCF-7 cell lines.


Fig3: Bioactive Peptide mixture cell-growth inhibiting effect on T47D cell lines.

Fig3: Bioactive Peptide mixture cell-growth inhibiting effect on T47D cell lines.


Fig 4. Cytotoxicity assay of the Tamoxifen on MDA-MB-435 cells.

Fig 4. Cytotoxicity assay of the peptide mixture on MDA-MB-435 cells. The plots show no negative effects towards the cell’s viability compared to the the negative control (Untreated, first bar to the left). The 6 concentrations as tested in the breast cancer anti-proliferation assay show all to be affecting the viability positively.


Fig 4b. Cytotoxicity assay of the Tamoxifen on MDA-MB-435 cells.

Fig 4b. Cytotoxicity assay of the Tamoxifen on MDA-MB-435 cells. The plots show a reduced viability reaching ~80% cell-count reduction at 10-5M and at 10-4M indicating toxic effects on the cells compared to the negative control (Untreated, first bar to the left). Accounting for that this is the central remedy in todays breast cancer therapy, a significant potential of the peptide mixture to reduced (perhaps abolish) side-effects and increase therapeutic strength is evident.


Effects of the peptide individually towards the breast cancer cell lines TMX2-28, MCF7 and T47D.


The sequence of the peptides is not disclosed. Table 1 below indicates the induced effect on the growth of the three cell lines, by the twelve peptides alone. The arrows indicated the overall effect at the 6 concentration dose-response assay. The arrows pointing down indicate cell-growth inhibition, while the arrows pointing upwards indicate cell-growth induction. The nature of action of the peptides alone and in mix is the cornerstone of future work on their function and which mechanisms they stimulate.


Table 1. The twelve peptides with their given names and their effect on the three breast cancer cell lines.

Table 1. The twelve peptides with their given names and their effect on the three breast cancer cell lines.


Following below is a figure 5 with plots from the dose-response assay of selected peptides of the twelve. Controls are: DMSO (contains cells + 0.001% DMSO), positive control BAP/Tamoxifen (contains cells + Benzo[a]pyrene or Tamoxifen) and negative control DC5 (contains only medium).

figure 5 with plots from the dose-response assay of selected peptides of the twelve.

PART II

Clinical investigation


Results I (human results)

The 18 human cases described represent a consecutive case series, of all human patients with benign or malignant tumours, seen between April 2003 and April 2004. They were not selected to show the best outcomes. Patients treated before April 22nd 2003 who returned for follow-up treatment or further examination within this period were included. Results are updated until April 2004.

Table 1: Summary of human treatment protocols and their interim outcomes

Table 1: Summary of human treatment protocols and their interim outcomes

Image of Table 1: Summary of human treatment protocols and their interim outcomes.

Results II (veterinary results)

The 16 veterinary cases described represent a consecutive case series, of all patients with benign or malignant tumours, seen between April 2003 and April 2004. They were not selected to show the best outcomes. Patients treated before April 22nd 2003 who returned for follow-up treatment or further examination within this period were included. Results are updated until April 2004.

Table 2: Summary of veterinary treatment protocols and their interim outcomes

Image of Table 1: Summary of veterinary treatment protocols and their interim outcomes.

Closing remarks and comments

On this investigation;
This investigation show one thing very clearly, and that is the ability of the body to heal itself. This ability is from time to time shown in the fact that most diseases, even cancer, may suddenly self-heal. This phenomenon is utterly disregarded by the medical society, as this show to the ability of all life to self heal. We just have to find what happen in the body that triggers this self healing ability. The authors have used acupuncture to trigger the healing, but acknowledge that quite many other modalities may act in the same way, as imaging, herbal medicine, meditation, osteopathy and surely many more.

In practical life there may be a number of factors that are able to trigger such a self healing. Therefore the medical society may be confused to what actually happen, and just label the phenomenon as “placebo”.

The authors of this article, however, regard the organism as a holistic entity, with multiple layers of action and feed-back control. Therefore an intervention on just one of these levels may trigger the complete and whole organism to respond. We have thus focused on one such level, the peptide level or mechanisms. Changes in the concentrations or presence of bio-active oligopeptides are an expression of a change in the whole organism, and will act also as a signalling force for the multitude of layers and levels in the body. The authors are convinced that such a change also may occur at other systems, and that the same may be found there, also inducing healing, but we have decided to concentrate on the peptides.

On the possibility of treating cancer with one needle acupuncture;
As the scientific investigations show that Thoresen's clinical results may be correct, then this opens the possibility to treat cancer with just one needle, or with the peptides emerging after this one needle. We then have two possibilities to treat, one expensive (injecting the peptides as a medical treatment), but also the cheap version of treating with acupuncture. This last possibility may show very important for areas of the world with many poor people.

On the action of acupuncture;
Acupuncture is usually considered to have its action through the nervous system, and many investigations and much thought is initiated to map out the working mechanisms here. This investigation however, shows that the blood may be of greater importance than we have thought. The whole action of acupuncture may in some cases be explained by the mere action of peptides in the blood, and these peptides are not produced by the nervous tissue, but by cutting parts of proteins already available in the blood stream. There are already some indications that this may be true. I will here mention the old medical knowledge that if the diseased area, muscle, joint or organ gets a good blood supply, then healing is often induced. Also the work of Dr. Björn Nordenström shows that the acupuncture meridians and points are more related to the blood vessels than the nerves, and this indicate the importance of the blood in the healing process.

On treating other diseases with acupuncture;
If cancer may be cured with such peptides, there are several diseases that also may be cured that way. The authors are presently engaged in mapping out this, and are working with such diseases as Horton headache, MS and several muscular and rheumatoid syndromes.

On the possibility of composing new medicines based on peptides;
As long as various diseases show a good reaction after acupuncture, even if this may be a minority of the patients, there will be the possibility to map out what peptides are active in the response and produce medicines from those. This will be a cheap and safe way to find new medicines, regarding the low possibility of harmful effects from the medicines, as they already are composed by the body itself.


References

  1. Thoresen A., Akupunkturbehandling av Equint Sarcoid, Norsk Veterinærtidsskrift, 1995 107, 10.
  2. Thoresen A, Veterinærmedisin, Komplementære og Alternative metoder, pp.442-445, ISBN 82-994172-4-4, 621 pages. English edition; ISBN 82-994172-2-8, gages 358-363.
  3. Coster S. and Fallowield L. J. (2002). The impact of endocrine therapy on patients with breast cancer: a review of the literature. The Breast, 11, 1-12
  4. Grabley S, Thiericke R. (1999). Bioactive agents from natural sources: trends in discovery and application. Adv Biochem Eng Biotechnol. 64:101-54. Review.
  5. DiMasi JA. (2002). The value of improving the productivity of the drug development process: faster times and better decisions. Pharmacoeconomics. 3:1-10.
  6. Gozgit et al., 2006 Arcaro lab.
  7. Wall, D.B., et al., Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry. Anal Chem, 2000. 72(6): p. 1099-111.
  8. Yan, F., et al., A comparison of drug-treated and untreated HCT-116 human colon adenocarcinoma cells using a 2-D liquid separation mapping method based upon chromatofocusing PI fractionation. Anal Chem, 2003. 75(10): p. 2299-308.
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